ABOUT THE LAB
The lab group focuses on studying the mechanisms of human RNA regulation in physiological processes and disease. In particular, it seeks to understand the class of RNA-binding proteins related to cancer and neurology.
In mechanistic terms, our group studies the elucidation of sets of mRNAs bound by specific proteins under a given cellular condition. We also elucidate the sets of proteins that participate in these complexes (interactome) using cellular models. In parallel, we produce recombinant proteins to employ them in biochemical methods and in crystallography to elucidate the mechanism of action of RNA-binding proteins. In addition, the group develops bioinformatics analyses for the area of RNA regulation.
The laboratory gave origin to the Center for Medicinal Chemistry at Unicamp (CQMED) of CBMEG, accredited as a Unit of the Brazilian Company for Industrial Research and Innovation of the Ministry of Science and Technology.
PRINCIPAL RESEARCHER
Dr. Katlin Brauer Massirer
Researcher and Coordinator
LINES OF RESEARCH
🧑🔬 RNA-binding proteins considered as therapeutic targets.
ACCREDITED GRADUATE PROGRAM
- Graduate Program in Genetics and Molecular Biology, University of Campinas (UNICAMP)
TEAM
SERVICES
Testing of reagents for molecular diagnostic experiments
- Production of recombinant proteins in eukaryotic and prokaryotic systems:
- Activity testing of recombinant proteins
- Biochemical/enzymatic assay for proteins
- Chemical synthesis of organic molecules
- Protein scanning in panel of molecules
- Derivation of stem cells
PUBLICATIONS
- Fanti, R. C., Vasconcelos, S. N. S., Catta-Preta, C. M. C., Sullivan, J. R., Riboldi, G. P., dos Reis, C. v., Ramos, P. Z., Edwards, A. M., Behr, M. A., & Couñago, R. M. (2022). A Target Engagement Assay to Assess Uptake, Potency, and Retention of Antibiotics in Living Bacteria. ACS Infectious Diseases. https://doi.org/10.1021/acsinfecdis.2c00073
- Lins, É. M., Oliveira, N. C. M., Reis, O., Ferrasa, A., Herai, R., Muotri, A. R., Massirer, K. B., & Bengtson, M. H. (2022). Genome-wide translation control analysis of developing human neurons. Molecular Brain, 15(1), 55. https://doi.org/10.1186/s13041-022-00940-9
- Wu, Qin, David Y. Nie, Wail Ba-alawi, YiShuai Ji, ZiWen Zhang, Jennifer Cruickshank, Jillian Haight, et al. 2022. “PRMT Inhibition Induces a Viral Mimicry Response in Triple-Negative Breast Cancer.” Nature Chemical Biology, May. https://doi.org/10.1038/s41589-022-01024-4.
- Segretti, Natanael D., Jéssica E. Takarada, Marcos A. Ferreira, André da Silva Santiago, Bruno V.M. Teodoro, Mariana C.F.C.B. Damião, Paulo H. Godoi, et al. 2022. “Discovery of Novel Benzothiophene Derivatives as Potent and Narrow Spectrum Inhibitors of DYRK1A and DYRK1B.” Bioorganic & Medicinal Chemistry Letters 68 (July): 128764. https://doi.org/10.1016/j.bmcl.2022.128764.
- Papes, Fabio, Antonio P. Camargo, Janaina S. de Souza, Vinicius M. A. Carvalho, Ryan A. Szeto, Erin LaMontagne, José R. Teixeira, et al. 2022. “Transcription Factor 4 Loss-of-Function Is Associated with Deficits in Progenitor Proliferation and Cortical Neuron Content.” Nature Communications 13 (1): 2387. https://doi.org/10.1038/s41467-022-29942-w.
- Ricardo, Ricardo A., André da Silva Santiago, Martin P. Schwalm, Zexi Hu, Caio v. dos Reis, Jessica E. Takarada, Priscila Mezzomo, et al. 2022. “Development of the First Covalent Monopolar Spindle Kinase 1 (MPS1/TTK) Inhibitor.” Journal of Medicinal Chemistry 65 (4). https://doi.org/10.1021/acs.jmedchem.1c01165.
- Scott, Fiona, Angela M. Fala, Jessica E. Takarada, Mihaela P. Ficu, Lewis E. Pennicott, Tristan D. Reuillon, Rafael M. Couñago, Katlin B. Massirer, Jonathan M. Elkins, and Simon E. Ward. 2022. “Development of Dihydropyrrolopyridinone-Based PKN2/PRK2 Chemical Tools to Enable Drug Discovery.” Bioorganic and Medicinal Chemistry Letters 60. https://doi.org/10.1016/j.bmcl.2022.128588.
- Eduful, Benjamin J., Sean N. O’Byrne, Louisa Temme, Christopher R. M. Asquith, Yi Liang, Alfredo Picado, Joseph R. Pilotte, et al. 2021. “Hinge Binder Scaffold Hopping Identifies Potent Calcium/Calmodulin-Dependent Protein Kinase Kinase 2 (CAMKK2) Inhibitor Chemotypes.” Journal of Medicinal Chemistry 64 (15): 10849–77. https://doi.org/10.1021/acs.jmedchem.0c02274.
- Ferreira, Tiago R., Rafael M. Couñago, and Nilmar S. Moretti. 2021. “Raising the Bar(-Seq) in Leishmania Genetic Screens.” Trends in Parasitology 37 (5): 367–69. https://doi.org/10.1016/j.pt.2021.03.002.
- Cabarca, Sindy, Maximilia Frazão de Souza, Andrew Albert de Oliveira, Gabriel S. Vignoli Muniz, M. Teresa Lamy, Caio Vinicius dos Reis, Jessica Takarada, et al. 2021. “Structure of the Mycobacterium Tuberculosis CPknF and Conformational Changes Induced in Forkhead-Associated Regulatory Domains.” Current Research in Structural Biology 3: 165–78. https://doi.org/10.1016/j.crstbi.2021.07.001.
CONTACT
Doctor Katlin Brauer Massirer
Researcher
Email: kmassire@unicamp.br
Group Photos
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